Since the number of observations in each sub-classwas unequal and disproportionate, the data wereanalysed by LSML91 version package of Harvey, 1990.The analysis of variance was carried out by model 1 ofHarvey [4], which had the facility to adjustsimultaneously for significant fixed by computingdifferent components of variance. To obtain theconstants for environmental effects, left hand sideconsisted of matrices of frequencies in differentsubclasses arranged in an m × n symmetrical matrix.The right hand side was the sum of dependent variablefor each subclass. The matrices were reduced to obtaina set of independent equations by imposingrestrictions. The reduced matrix was inverted. Theinverse elements were multiplied with right handmembers to obtain the least square constants for allthe levels of fixed effects.