cs Pure Swiss Hyaluronic Acid Serum can be applied in the morning and again at night time. Only apply a thin layer (less is more) and be sure to use a good moisturizer immediately afterward. Formulated to assist cell renewal and collagen formation, and designed to diminish the appearance of wrinkles and fine lines. You should expect to see results immediately, however we highly recommend to give the product at least 30 days to see the most optimal results.